rabbit polyclonal anti β casein  (Biorbyt)

Journal: Foods

Article Title: CRISPR/Cas9-Induced Knockout of miR-24 Reduces Cholesterol and Monounsaturated Fatty Acid Content in Primary Goat Mammary Epithelial Cells

doi: 10.3390/foods11142012

Figure Lengend Snippet: Knockout of miR-24 decreases miRNA abundance and increases INSIG1 and FASN abundance. ( A ) Abundance analysis of miR-24-3p by RT-qPCR in control and knockout cells. ( B ) Detecting the mRNA abundance of pri- and pre-miR-24 sequence in knockout cells. ( C ) Abundance analysis of miR-24-5p and miR-23b-3p in knockout cells. ( D ) mRNA abundance analysis of INSIG1 and FASN in control and knockout cells. ( E , F ) Abundance analysis of INSIG1 and FASN proteins in control and miR-24 knockout cells by Western blot. Data are shown as mean ± SEM in these repeated experiments. * p < 0.05, *** p < 0.001.

Article Snippet: The primary antibodies used were rabbit anti-β-casein (bs-0466R, Bioss, Woburn, CA; 1:1000), rabbit anti-INSIG1 (bs-5074R, Bioss; 1:500), rabbit anti-FASN (10624-2-AP, Proteintech Group, Wuhan, China; 1:500), and mouse anti-β-tubulin (CW0098, CW Biotech, Beijing, China; 1:1000).

Techniques: Knock-Out, Quantitative RT-PCR, Sequencing, Western Blot

Journal: Foods

Article Title: CRISPR/Cas9-Induced Knockout of miR-24 Reduces Cholesterol and Monounsaturated Fatty Acid Content in Primary Goat Mammary Epithelial Cells

doi: 10.3390/foods11142012

Figure Lengend Snippet: Knockout of miR-24 inhibits lipid droplet, TAG and cholesterol accumulation via targeting INSIG1 . ( A ) Abundance analysis of INSIG1 mRNA in miR-24 knockout cells transfected with siINSIG1 or siNC. ( B , C ) INSIG1 protein abundance analysis in miR-24 knockout cells transfected with siINSIG1 or siNC by Western blot. ( D – G ) The contents of lipid droplet ( D , E ), TAG ( F ) and cholesterol ( G ) in control and miR-24 knockout cells transfected with siINSIG1 or siNC. Data are expressed as mean ± SEM in these repeated experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The primary antibodies used were rabbit anti-β-casein (bs-0466R, Bioss, Woburn, CA; 1:1000), rabbit anti-INSIG1 (bs-5074R, Bioss; 1:500), rabbit anti-FASN (10624-2-AP, Proteintech Group, Wuhan, China; 1:500), and mouse anti-β-tubulin (CW0098, CW Biotech, Beijing, China; 1:1000).

Techniques: Knock-Out, Transfection, Western Blot

Journal: Foods

Article Title: CRISPR/Cas9-Induced Knockout of miR-24 Reduces Cholesterol and Monounsaturated Fatty Acid Content in Primary Goat Mammary Epithelial Cells

doi: 10.3390/foods11142012

Figure Lengend Snippet: Effects of INSIG1 knockdown on miR-24 KO-mediating changes in fatty acid composition.

Article Snippet: The primary antibodies used were rabbit anti-β-casein (bs-0466R, Bioss, Woburn, CA; 1:1000), rabbit anti-INSIG1 (bs-5074R, Bioss; 1:500), rabbit anti-FASN (10624-2-AP, Proteintech Group, Wuhan, China; 1:500), and mouse anti-β-tubulin (CW0098, CW Biotech, Beijing, China; 1:1000).

Techniques: